Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen
نویسندگان
چکیده
Background and Aim: The Kacang goat (Capra hircus) is an indigenous livestock species in Indonesia that at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected increase the population these goats. This study aimed determine addition epigallocatechin-3-gallate chitosan nanoparticles (EGCG CNPs) skim milk–egg yolk (SM–EY) extender obtain best possible quality post-thawed buck semen for AI. Materials Methods: Fresh was diluted SM–EY without or with 0.5, 1.0, 1.5, 2.0 µg EGCG CNPs/mL extender. Extended packaged French mini straws, frooze, stored liquid nitrogen −196℃ 24 hours. Six replicates from each treatment group were thawed catalase, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, malondialdehyde (MDA), sperm intact plasma membrane (IPM), viability motility analyses. Results: Post-thawed previously frozen CNPs (control group) exhibited lowest levels DPPH, living cells, motility, MPI, highest MDA. The T3 T4 groups increased (p < 0.05) decreased MDA than those T0 group. Meanwhile, MPI higher group. Conclusion: This first using extender, which adding 1.5 μg/mL this antioxidant capacity semen.
منابع مشابه
Quality of Bovine Chilled or Frozen-Thawed Semen after Addition of Omega-3 Fatty Acids Supplementation to Extender
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ژورنال
عنوان ژورنال: F1000Research
سال: 2023
ISSN: ['2046-1402']
DOI: https://doi.org/10.12688/f1000research.127744.2